- Note: This list is not yet complete.
Star Trek: The Original Series
- Montgomery Scott
- "The Cage"
- "The Man Trap" (voice only)
- "Charlie X"
- "What Are Little Girls Made Of?"
- "Miri"
- "Dagger of the Mind"
- "The Menagerie, Part II"
- "The Conscience of the King"
- "Shore Leave"
- "Court Martial"
- "This Side of Paradise"
- "Errand of Mercy"
- "The Alternative Factor"
- "Amok Time"
- "Journey to Babel" (mentioned only)
- "The Omega Glory"
- "All Our Yesterdays" (voice only)
- Hikaru Sulu
- "The Cage"
- "Charlie X"
- "What Are Little Girls Made Of?"
- "Miri"
- "Dagger of the Mind"
- "The Conscience of the King"
- "Court Martial"
- "Space Seed"
- "A Taste of Armageddon"
- "The Devil in the Dark"
- "The Alternative Factor"
- "The Apple"
- "Journey to Babel"
- "Obsession"
- "The Trouble with Tribbles"
- "The Gamesters of Triskelion"
- "A Piece of the Action"
- "A Private Little War"
- "Patterns of Force"
- "By Any Other Name"
- "Bread and Circuses"
- "Plato's Stepchildren"
- "Requiem for Methuselah"
- "All Our Yesterdays"
Star Trek: The Animated Series
- Arex
- "Beyond the Farthest Star" (no lines)
- "Yesteryear"
- "More Tribbles, More Troubles"
- "The Survivor"
- "The Magicks of Megas-tu"
- "The Time Trap"
- "The Slaver Weapon"
- "The Jihad"
- Christine Chapel
- "Beyond the Farthest Star"
- "Yesteryear"
- "One of Our Planets Is Missing"
- "More Tribbles, More Troubles"
- "The Infinite Vulcan"
- "The Magicks of Megas-tu"
- "Once Upon a Planet"
- "The Time Trap"
- "The Slaver Weapon"
- "The Eye of the Beholder"
- "The Jihad"
- TAS Season 2
- James T. Kirk
- M'Ress
- "Beyond the Farthest Star"
- "Yesteryear"
- "One of Our Planets Is Missing"
- "The Lorelei Signal"
- "More Tribbles, More Troubles"
- "The Infinite Vulcan"
- "The Magicks of Megas-tu"
- "The Terratin Incident"
- "The Time Trap"
- "The Ambergris Element"
- "The Slaver Weapon"
- "The Jihad"
- "The Pirates of Orion"
- "Albatross"
- "How Sharper Than a Serpent's Tooth"
- "The Counter-Clock Incident"
- Leonard H. McCoy
- "The Slaver Weapon"
- "Bem"
- Montgomery Scott
- Hikaru Sulu
- Uhura
- "Yesteryear"
- "The Survivor" (appeared in recycled animation only)
- "Mudd's Passion" (recycled animation only)
- "The Ambergris Element" (no lines)
- "The Eye of the Beholder"
- "The Jihad"
- "The Pirates of Orion"
Star Trek: The Next Generation
- Worf
- "Code of Honor"
- "Haven"
- "Shades of Gray" (in stock footage only)
- "Thine Own Self" (only as a hologram)
- Geordi La Forge
- "A Matter of Honor"
- "Manhunt"
- "First Contact"
- "Redemption, Part I" (no lines)
- "Tapestry" (only voice)
- "Attached"
- "Thine Own Self" (only as a hologram)
- Deanna Troi
- "Hide and Q"
- "Datalore"
- "11001001"
- "Heart of Glory"
- "A Matter of Honor"
- "Yesterday's Enterprise" (no lines)
- "Identity Crisis"
- "Redemption, Part I" (no lines)
- "The Perfect Mate"
- "The Next Phase" (no lines)
- "The Inner Light"
- "Relics" (no lines)
- Beverly Crusher
- TNG Season 2 except for:
- "Shades of Gray" (stock footage)
- "The Wounded"
- "Redemption, Part I" (no lines)
- "Cost of Living" (no lines)
- TNG Season 2 except for:
- Wesley Crusher
- "The Last Outpost"
- "Haven"
- "Too Short a Season"
- "Heart of Glory"
- "The Arsenal of Freedom"
- "We'll Always Have Paris"
- "Conspiracy"
- "The Neutral Zone"
- "Elementary, Dear Data"
- "Time Squared"
- "Up the Long Ladder"
- "The Emissary"
- "Shades of Gray" (in stock footage only)
- "Who Watches the Watchers?"
- "The Defector"
- "Deja Q"
- "Captain's Holiday"
- "Legacy"
- "Future Imperfect"
- TNG Season 4 after "Final Mission"
- TNG Season 5 except for:
- TNG Season 6
- TNG Season 7 except for:
- Tasha Yar
- "We'll Always Have Paris"
- "Conspiracy"
- "The Neutral Zone"
- TNG Season 2 except for:
- "Shades of Gray" (stock footage only)
- TNG Season 3 except for:
- TNG Season 4
- TNG Season 5
- TNG Season 6
- TNG Season 7 except for:
Star Trek: Deep Space Nine
- Kira Nerys
- "Through the Looking Glass" (only as Kira Nerys (mirror))
- "The Assignment"
- "Let He Who Is Without Sin..."
- "The Emperor's New Cloak" (only as Kira Nerys (mirror))
- Julian Bashir
- "Paradise"
- "Blood Oath"
- "Through the Looking Glass" (only as Julian Bashir (mirror))
- "For the Uniform"
- Jadzia Dax
- "Through the Looking Glass" (only as Jadzia Dax (mirror))
- "The Begotten"
- "In the Cards"
- "Inquisition" (only as a hologram)
- DS9 Season 7
- Odo
- "Paradise"
- "By Inferno's Light"
- "Far Beyond the Stars" (only as Douglas Pabst)
- "Change of Heart"
- "Strange Bedfellows" (only as a Pah-wraith posing as Odo)
- Worf
- DS9 Season 1
- DS9 Season 2
- DS9 Season 3
- "Rocks and Shoals"
- "Inquisition" (only as a hologram)
- "Chrysalis"
- "The Emperor's New Cloak" (only as Worf (mirror))
- Miles O'Brien
- "Dax"
- "The Passenger"
- "Move Along Home"
- "Necessary Evil"
- "Blood Oath"
- "Equilibrium"
- "Second Skin"
- "Through the Looking Glass" (only as Miles O'Brien (mirror))
- "Indiscretion"
- "Let He Who Is Without Sin..."
- "Things Past"
- "Far Beyond the Stars" (only as Albert Macklin)
- "In the Pale Moonlight"
- "The Reckoning"
- "Valiant"
- "Profit and Lace"
- "The Emperor's New Cloak" (only as Miles O'Brien (mirror))
- Quark
- "Past Prologue"
- "Battle Lines"
- "Paradise"
- "Crossover" (only as Quark (mirror))
- "Tribunal"
- "Second Skin"
- "Past Tense, Part II"
- "Far Beyond the Stars" (only as Herbert Rossoff)
- "Waltz"
- "Inquisition" (only as a hologram)
- "Inter Arma Enim Silent Leges"
- Jake Sisko
- "Past Prologue"
- "Q-Less"
- "The Circle"
- "Armageddon Game"
- "Paradise"
- "Blood Oath"
- "The Wire"
- "Crossover"
- "Tribunal"
- "The House of Quark"
- "Second Skin"
- "Defiant"
- "Past Tense, Part I"
- "Past Tense, Part II"
- "Destiny"
- "Visionary"
- "Through the Looking Glass"
- "The Sword of Kahless"
- "To the Death"
- "Trials and Tribble-ations"
- "The Darkness and the Light"
- "Resurrection"
- "The Magnificent Ferengi"
- "Waltz"
- "One Little Ship"
- "Change of Heart"
- "Wrongs Darker Than Death or Night"
- "Inquisition"
- "In the Pale Moonlight"
- "Once More Unto the Breach"
- "The Emperor's New Cloak"
- "Badda-Bing, Badda-Bang"
- "Inter Arma Enim Silent Leges"
- "Extreme Measures"
Star Trek: Voyager
Technically, "Living Witness" had none of the main characters except for The Doctor (or his back-up).
- Kes
- "Non Sequitur"
- "Maneuvers"
- "Resistance"
- VOY Season 4 except for:
- VOY Season 5
- VOY Season 6 except for:
- "Fury"
- VOY Season 7
Star Trek: Enterprise
Since "In a Mirror, Darkly" and "In a Mirror, Darkly, Part II" only featured mirror-versions of the main characters, none of the actual main characters appeared, except for mirror Archer's hallucination of Jonathan Archer in the second part.
- Malcolm Reed
- "Acquisition" (no lines)
- "Carbon Creek"
- "First Flight"
- "Carpenter Street" (voice only)
- Phlox
- "Acquisition" (no lines)
- "Carbon Creek"
- "First Flight"
- "Carpenter Street" (mentioned only)
- "Proving Ground"
- "Awakening"
- Hoshi Sato
- "Acquisition" (no lines)
- "Carbon Creek"
- "Carpenter Street"
- Travis Mayweather
- "Acquisition" (no lines)
- "Carbon Creek"
- "Cogenitor"
- "First Flight"
- "Carpenter Street"
- "Doctor's Orders" (no lines)