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Nitrous oxide (N2O) is a colorless gas that often made Humans insensible to pain and caused spontaneous laughter; due to this effect, it was often dubbed laughing gas. In sufficient concentrations, it was a potential hallucinogen, though these concentrations were potentially lethal due to the extreme displacement of oxygen rendering the air unbreathable. (LD: "Room for Growth")

In 2270, when the computer circuits of the USS Enterprise were affected by subatomic particles from an energy field encountered by the ship and began playing practical jokes on the crew, among its tricks was to fill the ship with nitrous oxide.

After a few minutes of exposure to nitrous oxide, a Vulcan would lose consciousness, as their physiology was unable to process the effects of the gas. (TAS: "The Practical Joker")

Orions were immune to the effects of nitrous oxide. (LD: "Room for Growth")

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