Charlene Masters
Gender:	Female
Species:	Human
Affiliation:	Starfleet
Rank:	Lieutenant
Occupation:	Sciences division officer
Status:	Alive (2267)
Played by:	Janet MacLachlan

Lieutenant Charlene Masters was a Starfleet Sciences division officer with engineering responsibilities aboard the Enterprise in 2267.

Masters was in charge of re-amplifying the starship's dilithium crystals after a near-collapse of the known universe drained them of power and put the ship at risk. Identical beings from opposite universes each desired the crystals for their own purposes. Anti-Lazarus rendered her unconscious and stole a pair of crystals as part of his plan to prevent his mad opponent, Lazarus, from destroying both of their universes. Later, Lazarus created a fire to force her out of engineering to steal his own dilithium.

Along with her assistant, Masters shared a low opinion of Enterprise coffee. (TOS: "The Alternative Factor")